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Elastase, powder

Pancreatic Elastase is a serine protease with specificity for peptide bonds adjacent to neutral amino acids. It also exhibits esterase and amidase activity. While Elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. It is produced in the pancreas as an inactive zymogen, proelastase, and activated in the duodenum by trypsin. Elastase is also found in blood components and bacteria.

Elastin is found in highest concentration in the elastic fibers of connective tissues; therefore, elastase is frequently used to dissociate tissues that contain a large amount of connective tissue. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin. Elastase is the enzyme of choice for the isolation of Type II cells from the lung.

Elastase has a molecular weight of 25,900 dalton, and an optimum pH for enzymatic activity of 8.5. It is unstable at pH 3.5.

Source: Porcine pancreas
2X crystallized; dialyzed and lyophilized
Activity: 3 units per mg protein
One unit cleaves 1 micromole of SucAla3NA per minute at 25C, pH 8.0.

Technical Information

• SDS - Elastase, powder
Catalog No. Size Price Lot # QTY
I52193 25 mg 257.50
I52110 100 mg 470.00
I52101 1 gm 2450.00
Enter Lot # if you require a specific lot, otherwise leave blank. 

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